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Image Search Results
Journal: PLoS ONE
Article Title: Clathrin Assembly Protein CALM Plays a Critical Role in KIT Signaling by Regulating Its Cellular Transport from Early to Late Endosomes in Hematopoietic Cells
doi: 10.1371/journal.pone.0109441
Figure Lengend Snippet: (A) WT and CALM −/− murine embryonic fibroblasts (MEFs) engineered to express KIT were incubated with biotinylated SCF for 60 min, and then with the APC-conjugated streptavidin for 30 min at 4°C. After stripping unincorporated SCF, the amount of the internalized SCF-KIT complex was quantified from the fluorescence intensity at the indicated times. (B) Uptake of SCF in WT or CALM −/− MEFs. The vertical axis indicates the ratio of mean fluorescence intensity, MFI (internalized SCF-KIT complex/initial surface KIT) (Data represent means ± SD, n = 3, n.s.: not significant (p = 0.079)).
Article Snippet: Then, MNCs were incubated with the antibodies (Abs) as follows: anti-lineage Abs (a cocktail of biotinylated Abs against CD3e (145–2C11), CD45R/B220 (RA3–6B2), Gr-1 (RB6–8C5), and TER-119 (TER-119)), fluorescein isothiocyanate (FITC)-conjugated anti-Sca-1 Ab (D7), allophycocyanin (APC)-conjugated anti-c-KIT Ab (2B8), and
Techniques: Incubation, Stripping Membranes, Fluorescence
Journal: PLoS ONE
Article Title: Clathrin Assembly Protein CALM Plays a Critical Role in KIT Signaling by Regulating Its Cellular Transport from Early to Late Endosomes in Hematopoietic Cells
doi: 10.1371/journal.pone.0109441
Figure Lengend Snippet: Localization of KIT was analyzed before and after SCF stimulation under confocal microscopy using WT and CALM −/− MEFs engineered to express KIT. KIT was visualized by the biotinylated anti-KIT antibody (Ab) and AlexaFluor 568 streptavidin conjugates.
Article Snippet: Then, MNCs were incubated with the antibodies (Abs) as follows: anti-lineage Abs (a cocktail of biotinylated Abs against CD3e (145–2C11), CD45R/B220 (RA3–6B2), Gr-1 (RB6–8C5), and TER-119 (TER-119)), fluorescein isothiocyanate (FITC)-conjugated anti-Sca-1 Ab (D7), allophycocyanin (APC)-conjugated anti-c-KIT Ab (2B8), and
Techniques: Confocal Microscopy